Molecular Identification and Immunity Functional Characterization of Lmserpin1 in Locusta migratoria manilensis
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چکیده
منابع مشابه
Novel Bacillus thuringiensis δ-endotoxin active against Locusta migratoria manilensis.
A novel δ-endotoxin gene was cloned from a Bacillus thuringiensis strain with activity against Locusta migratoria manilensis by PCR-based genome walking. The sequence of the cry gene was 3,432 bp long, and it encoded a Cry protein of 1,144 amino acid residues with a molecular mass of 129,196.5 kDa, which exhibited 62% homology with Cry7Ba1 in the amino acid sequence. The δ-endotoxin with five c...
متن کاملFunctional interaction of nicotinic acetylcholine receptors and Na+/K+ ATPase from Locusta migratoria manilensis (Meyen)
Associated proteins are important for the correct functioning of nicotinic acetylcholine receptors (nAChRs). In the present study, a neonicotinoid-agarose affinity column was used to isolate related proteins from a solubilized membrane preparation from the nervous system of Locusta migratoria manilensis (Meyen). 1530 peptides were identified and most of them were involved in the membranous stru...
متن کاملDetermination of Trace Level of cAMP in Locusta Migratoria Manilensis Meyen by HPLC with Fluorescence Derivation
A sensitive and rapid method was developed for the determination of cAMP in Locusta migratoria manilensis Meyen by high-performance liquid chromatography with fluorescence detection. The cAMP was derivatized using chloroacetaldehyde and TBAS buffer/methanol was used as the mobile phase. A detection quantification of 40 fmol/ml could be achieved when using fluorescence detection. An HPLC-MS meth...
متن کاملMolecular and Functional Characterization of cDNAs Putatively Encoding Carboxylesterases from the Migratory Locust, Locusta migratoria
Carboxylesterases (CarEs) belong to a superfamily of metabolic enzymes encoded by a number of genes and are widely distributed in microbes, plants and animals including insects. These enzymes play important roles in detoxification of insecticides and other xenobiotics, degradation of pheromones, regulation of neurodevelopment, and control of animal development. In this study, we characterized a...
متن کاملIdentification of Representative Genes of the Central Nervous System of the Locust, locusta migratoria manilensis by Deep Sequencing
The shortage of available genomic and transcriptomic data hampers the molecular study on the migratory locust, Locusta migratoria manilensis (L.) (Orthoptera: Acrididae) central nervous system (CNS). In this study, locust CNS RNA was sequenced by deep sequencing. 41,179 unigenes were obtained with an average length of 570 bp, and 5,519 unigenes were longer than 1,000 bp. Compared with an EST da...
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ژورنال
عنوان ژورنال: Insects
سال: 2021
ISSN: 2075-4450
DOI: 10.3390/insects12020178